Oligonucleotide Purification using Ion Pair Reversed-Phase Liquid Chromatography

Purifying synthetic oligonucleotides – especially longer sequences – poses unique challenges. Agilent’s PLRP-S columns and ion-pair reversed-phase chromatography workflows are designed to help you overcome these hurdles with precision and scalability.

This guide covers best practices for buffer selection, pore size optimisation and transition from analytical to preparative workflows. If you’re producing oligos for therapeutics or diagnostics, this is an essential resource to achieve high-purity outcomes at any scale.

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