Oligonucleotide analysis using SFC

Discover a novel approach for oligonucleotide impurity analysis using supercritical fluid chromatography (SFC). This application note highlights how SFC, combined with Shim-pack UC-Diol II columns and optimised modifiers, enables high-resolution separation of short-chain oligonucleotides, including challenging phosphorothioate (PS) impurities.

Compared with conventional methods, SFC offers unique selectivity and improved peak shapes for various sequences. If you’re seeking advanced solutions for oligonucleotide therapeutic quality control, download now to learn how SFC can enhance your analytical capabilities and support future research into longer oligonucleotides.

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