LC/MS Oligonucleotide Analysis with Altura HPH-C18

The LC/MS analysis of oligonucleotides typically requires the use of alkylamine-based ion-pairing agents to achieve adequate retention and peak shape.

Learn how the Agilent workflow captures a sufficient ion set to support robust deconvolution and precise molecular weight confirmation of the target oligonucleotides. This application note demonstrates how the Altura Oligo HPH-C18 column, combined with the InfinityLab Pro iQ mass detector and 1260 Infinity III Prime Bio LC system, enables high-resolution separations under MS-friendly conditions.

See how ultra-inert column hardware minimizes secondary interactions, improves detection of challenging n-1 impurities, and delivers reliable impurity profiling and characterization workflows for therapeutic oligonucleotides.

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